| Reagent | Final concentration | Amount |
|---|---|---|
| Supplemented KnockOutTM DMEM-medium | - | 8.925 mL |
| Collagenase II | 0.1% (w/v) | 10 mg |
| Collagenase IV | 0.25% (w/v) | 25 mg |
| Dispase | 0.25 U/mL | 1 ml (from 2.5 U/mL stock solution prepared according to manufacturer's instructions) |
| DNAse I | 7.5 μg/mL | 75 μL (from 1 mg/mL stock solution prepared according to manufacturer's instructions) |
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