Reagent | Final concentration | Amount |
---|---|---|
Supplemented KnockOutTM DMEM-medium | - | 13.39 mL |
Collagenase I | 0.1% (w/v) | 15 mg |
Collagenase II | 0.1% (w/v) | 15 mg |
Dispase | 0.25 U/mL | 1.5 mL (from 2.5 U/mL stock solution prepared according to manufacturer's instructions) |
DNAse I | 7.5 μg/mL | 112.5 μL (from 1 mg/mL stock solution prepared according to manufacturer's instructions) |