Skip to main content
. 2021 May 1;2(2):100489. doi: 10.1016/j.xpro.2021.100489
Reagent Final concentration Amount
Supplemented KnockOutTM DMEM-medium - 13.39 mL
Collagenase I 0.1% (w/v) 15 mg
Collagenase II 0.1% (w/v) 15 mg
Dispase 0.25 U/mL 1.5 mL (from 2.5 U/mL stock solution prepared according to manufacturer's instructions)
DNAse I 7.5 μg/mL 112.5 μL (from 1 mg/mL stock solution prepared according to manufacturer's instructions)