Fig. 4.

Methods of human tumour cryopreservation maintain biological pathways. a Euler diagrams highlighting the overlaps between gene ontology (GO) pathways detected in FT clusters and cryopreserved replicates from CCS, CT and CO. A total of 315, 347, 368, 262, 230 and 311 pathways were assessed from the FT replicates across the BC-P1, BC-P2, BC-P3, PC-P1, PC-P2 and M-P1 cases, respectively. b–d Sensitivity of pathway enrichment scores detected in clusters across cryopreserved replicates of BC-P1 (b), PC-P1 (c) and M-P1 (d). The minimum, mean and maximum -log10 q value are plotted in the error bars of each GO pathway. All DEGs from each cluster were passed on to the ClusterProfiler package for functional enrichment with the CC sub-ontology under the human org. Hs.eg.db database. GO pathway descriptions can be found in Additional file 4