Substrate specificity of Tt27-HBDH.
| K M (mM) | k cat (s−1) |
k
cat/KM (M−1 s−1) |
|
|---|---|---|---|
| EAA | 325.5 | 21.1 | 64.9 |
| (S)-EHB | 343 | 7.33 | 21.4 |
| NADH | 0.0334 | 20.3 | 606 660 |
| NAD+ | 0.186 | 12.9 | 69 400 |
| Ethyl 2-chloroacetoacetate | 144.6 | 143.5 | 990 |
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