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. 2021 May 11;22:150. doi: 10.1186/s13059-021-02343-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Super-resolution 3D imaging of chromosome regions. a Widefield 3D image of a single fluorescently labeled chromatin region (maximum intensity projection of a z-stack). b,c Super-resolution 3D images of the same structure obtained with ZOLA-3D [35]. b A color-coded view, where color indicates axial coordinate. c Two 500-nm-thick slices through the 3D super-resolution image are shown, with two 1 μm × 1 μm regions (yellow boxes) shown magnified to the right. The white arrow points to an isolated piece of chromatin fiber. For an animated 3D view, see Additional file 2: Video S1