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. 2021 May 11;19:134. doi: 10.1186/s12951-021-00877-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — In vitro ¹O₂ detection of Lipo/ICG a and Lipo/pB-DOX/ICG b (containing 15 µg/mL ICG) using ¹O₂ probe DPBF, whose attenuation rate at 407 nm is directly proportional to the generation rate of ¹O₂ under 808 nm laser radiation (1.0 W/cm², 300 s). c Temperature rise profiles of PBS, free ICG, Lipo/pB-DOX, Lipo/ICG, and Lipo/pB-DOX/ICG groups, all containing 12.5 µg/mL ICG, and PBS group under 808 nm laser irradiation (1.0 W/cm², 5 min ). d Temperature rise profile of Lipo/pB-DOX/ICG with different ICG concentrations (ranging from 0 to 50 µg/mL) under 808 nm laser irradiation (1.0 W/cm², 5 min ). e Infrared thermographic maps of Lipo/pB-DOX/ICG (12.5 µg/mL) at different time points under 808 nm laser irradiation (1.0 W/cm²)