Table 3.
Plasmids used in this study
| Plasmid | Relevant characteristics | References |
|---|---|---|
| pEC-XT99A | TetR, C. glutamicum/E. coli shuttle vector (Ptrc, lacIq, pGA1 oriVCg) | [74] |
| pEC-XT99A-gabTD | pEC-XT99A expressing gabT and gabD from P. stutzeri ATCC 17588 | [16] |
| pEC-XT99A-gabTDP134L | pEC-XT99A expressing gabT and gabD with SNP P134L from P. stutzeri ATCC 17588 | [73] |
| pEC-XT99A-tetA(Z)Δ21bp-gabTDP134L | pEC-XT99A with 21 bp off-frame deletion in tetA(Z) expressing gabT and gabD with SNP P134L from P. stutzeri ATCC 17588 | This study |
| pEKEx3 | SpecR, C. glutamicum/E. coli shuttle vector (Ptac lacIq pBL1, oriVEc) | [75] |
| pEKEx3-patDA | pEKEx3, expressing patD and patA from E. coli MG1655 | [16] |
| pEKEx3-gltBD | pEKEx3, expressing gltBD from C. glutamicum ATCC 13032 | This study |
| pEKEx3-gltBE686QD | pEKEx3, expressing gltBD with an amino acid exchange gltBE686Q from C. glutamicum ATCC 13032 | This study |
| pVWEx1 | KanR, C. glutamicum/E. coli shuttle vector (Ptac, lacIq) | [76] |
| pVWEx1-ldcC | pVWEx1 expressing ldcC from E. coli MG1655 | [77] |
| pK19mobsacB | KanR, mobilizable E. coli vector mutagenesis (oriV, sacB) | [78] |
| pK19mobsacB-gltBE686Q | pK19mobsacB to introduce SNP gltBE686Q | This study |
| pK19mobsacB-ΔgltB | pK19mobsacB for deletion of gltB from C. glutamicum ATCC 13032 | This study |