Fig. 8.

The inhibition of Gm13568 in astrocytes decreases Notch1 pathway activation and ameliorates the pathological process in EAE mice. On day 7 after administration with LV-ctrl or LV-Inhibit Gm13568, the mice were immunized with MOG_35-55 for 23 days. a The clinical scores for EAE mice infected by LV-ctrl or LV-Inhibit Gm13568 (n = 10 mice per group). b The expression of IL-9 and GFAP and the activation of Notch1 pathway in the spinal cords were evaluated by Western blot assay. The results of a typical experiment are presented. c, d The levels of IL-6, TNF-α, and IP-10 from the spinal cords and peripheral blood in lentivirus-infected mice were analyzed by real-time PCR and CBA assay, respectively. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. PBS group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001versus LV-ctrl group (one-way ANOVA, the two-tailed Student’s t test). e H&E staining for inflammatory cells infiltrations in spinal cords of mice (Scale bars, 50 μm). f LFB staining for the medullary sheath damage in spinal cords of mice (scale bars, 50 μm). Boxed areas in the upper rows are presented enlarged underneath