Fig. 3.

Effects of oxycodone on placental gene expression in female and male mouse conceptuses. A) 3D PCA plot of RNAseq results from placenta of OXY females vs. CTL females. B) Heatmap based on all transcripts identified in placenta of OXY females vs. CTL females d. C) Volcano plot analyses of gene transcripts identified in placenta of OXY females vs. CTL females. Transcripts represented by Orange: p < 0.05; Transcripts represented by Red: log2FoldChange < −1 or > 1; Transcripts represented by Green: p < 0.05 AND log2FoldChange < −1 or > 1. D) 3D PCA plot of RNAseq results from placenta of OXY males vs. CTL males. E) Heatmap based on all transcripts identified in placenta of OXY males vs. CTL males. F) Volcano plot analyses of gene transcripts identified in placenta of OXY males vs. CTL males. Transcripts represented by Orange: p < 0.05; Transcripts represented by Red: log2FoldChange < −1 or > 1; Transcripts represented by Green: p < 0.05 AND log2FoldChange < −1 or > 1. For the CTL group, 10 females and 10 males were initially tested, and for the OXY group, 11 females and 11 males were initially tested. However, the PCA analysis revealed that one OXY female and one CTL female placenta were outliers. These samples were thus not considered in subsequent analyses. Likewise, this analysis revealed that 3 OXY male placentas were outliers and removed from further RNAseq analyses.