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. 2021 May 11;22:28. doi: 10.1186/s40360-021-00496-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The effect of GA on LPS-induced WI-38 apoptosis and inflammation.aWI-38 cells were cultured in vitro (Ctrl), or treated with 10mg/ml LPS for 24h (LPS), or pre-incubated with 50 nM GA for 24h prior to LPS treatment (LPS + GA). A TUNEL assay was applied to identify apoptotic cells with TUNEL (Red) immunoreaction. During the meantime, DAPI (Blue) immunoreaction was applied to identify WI-38 cell nuclei. bFor images acquired in TUNEL assay, the percentages of apoptotic WI-38 cells were compared among Ctrl, LPS and LPS + GA conditions (* P < 0.05). cFor WI-38 cells under Ctrl, LPS and LPS + GA conditions, western blot analysis were conducted to compare IL-6 and MCP-1 protein expressions. dFor western blot data in (c), relative band intensities of IL-6 and MCP-1 were compared between LPS and LPS + GA conditions (* P < 0.05)