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. 2021 May 11;22:28. doi: 10.1186/s40360-021-00496-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effect of Akt knockdown in WI-38 cells double-treated with GA and LPS. aWI-38 cells were transfected with a siRNA specifically targeting human Akt gene (si_Akt), and a non-specific control siRNA (si_C). Then, a qRT-PCR assay was performed to compare WI-38 endogenous Akt expressions (* P < 0.05). bCell viabilities was compared among WI-38 cells under control (ctrl) condition (not transfected by siRNA, nor treated with LPS), WI-38 cells transfected with si_C or si_Akt, and siRNA-transfected WI-38 cells treated with 10mg/ml LPS for 24h (* P < 0.05; ∆ P > 0.05). cSiRNA-transfected WI-38 cells were pre-incubated with 50 nM GA for 24h and then treated with 10mg/ml LPS for another 24h (LPS + GA). A TUNEL assay was applied to identify apoptotic cells with TUNEL (Red) immunoreaction. Also, DAPI (Blue) immunoreaction was applied to identify WI-38 cell nuclei. dFor siRNA-transfected WI-38 cells which were treated with LPS + GA, the percentages of apoptotic WI-38 cells were compared (* P < 0.05). eFor siRNA-transfected WI-38 cells which were treated with LPS + GA, western blot analysis was conducted to examine IL-6 and MCP-1 protein expressions. fFor western blot data in (E), quantitative assessment on relative band intensities of IL-6 and MCP-1 were performed (* P < 0.05)