Fig. 2.
In vitro enhancer classification is preserved in clinical samples. a Normalized ChIPseq of AR (n = 87), H3K27ac (n = 92), and H3K27me3 (n = 76) from primary PCa samples. A significant enrichment of AR and H3K27ac is observed at inducible and constitutive ARBS compared to inactive enhancers (ns > 0.05, **p < 10−4, ***p < 10−6). H3K27me3 was also significantly enriched at inactive enhancers compared to inducible or constitutive enhancers. b H3K27ac ChIPseq was done in 4 patients with matched PCa tissue pre- and post-enzalutamide treatment. The box plots show the normalized H3K27ac enrichment ±2 kb around induced (red), constitutive (dark gray), and inactive (gray) ARBS enhancers. c H3K27ac enrichment in each class of enhancers was normalized within each tumor and compared before and after ENZA treatment. H3K27ac enrichment at induced enhancers was markedly reduced after ENZA treatment