Figure 7. RT-qPCR and Western blot to detect the expression of TGF-beta/Smad pathway related molecules. (a) RT-qPCR to assay TGF-β1, Smad2, and Smad3 gene expression; (b–c) Western blot to assay TGF-β1, Smad2, and Smad3 protein expression. **p < 0.01 vs. control group; ##p < 0.01 vs. model group; MH: mild hypothermia; MH+Rutin: mild hypothermia plus rutin.
