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. 2021 Apr 22;22(1):664. doi: 10.3892/etm.2021.10096

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Regulatory effect of miR-770-5p on BAG5 expression in Min6 cells. Min6 cells were transfected with control-siRNA, BAG5-siRNA, inhibitor control, miR-770-5p inhibitor or BAG5-siRNA + miR-770-5p inhibitor for 48 h. (A) Transfection efficiency of miR-770-5p inhibitor. The transfection efficiency of BAG5-siRNA was assessed via (B) RT-qPCR and (C) western blotting. BAG5 (D) mRNA and (E) protein expression levels were determined via RT-qPCR and western blotting, respectively. ^**P<0.01 vs. control; ^##P<0.01 vs. inhibitor. miR, microRNA; BAG5, Bcl-2 associated athanogene 5; siRNA, small interfering RNA; RT-qPCR, reverse transcription-quantitative PCR.