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. 2021 Apr 23;44(4):245–253. doi: 10.14348/molcells.2021.0037

Fig. 1. Generation of AMM/S cell line using TALEN gene editing.

Fig. 1

(A) Schematic diagram of the donor vector carrying TGF-β1-bearing donor plasmid DNA. An expression cassette containing the PGK promoter-driven TGF-β1 and EF1α promoter-driven GFP-T2A-puromycin was inserted into the AAVS1 site via homology-directed repair. The locations of primers for junction detection are indicated (primers F and R). HA-L, left homology arm; HA-R, right homology arm; PGK, phosphoglycerate kinase promoter; EF1α, elongation factor-1 alpha promoter; Puro, puromycin. (B) Inserted donor plasmid was confirmed using junction PCR. (C) GFP-expressing AMM/S. Transfected cells were selected using puromycin followed by FACS. Scale bars = 500 μm. (D) Expression levels of TGF-β1 were examined using qPCR. **P < 0.01, n = 4.