Fig. 5. Hsd17b4 is unlikely to be involved in efferocytosis.

(A and B) LR73 cells transfected with the indicated plasmids were incubated with TAMRA-labeled apoptotic thymocytes for 2 h, extensively washed with ice-cold PBS, trypsinized, and analyzed by flow cytometry. Cells double-positive for GFP and TAMRA were considered to be phagocytes engulfing apoptotic cells. NS, not significant. (C) Schematic diagram of the assay used to assess continuous efferocytosis. (D and E) LR73 cells transfected with the indicated plasmids were incubated with the same number of unstained apoptotic cells. After incubation for 24 h, LR73 cells were incubated with TAMRA-stained apoptotic cells for 2 h, and then LR73 cells engulfing TAMRA-stained apoptotic cells were analyzed by flow cytometry.