Fig. 7. MT1M is involved in SOD1/PI3K signaling pathway.
(A) SOD1 inhibitor LD100 as well as ZnSO4 were used, re-expressing of MT1M can further enhance the down-regulative effects of inhibitor LD100 on the SOD1/PI3K-AKT pathway, while Zinc supplementation induced MT1M expressing and suppressed SOD1 and its downstream targets. (B) Quantitative analysis of western blots (*P < 0.05, **P < 0.01). (C) MT1M downregulated SOD1 expression by Immuno-fluorescence microscopy. MT1M stained red, which was hardly seen in the vector-transfected cells. SOD1 stained green mainly in the in the cytoplasm and nucleus membrane. (D) Schematic representation of the possible pathogenic mechanism of MT1M/SOD1 axis in ESCC progression.