Fig. 3.

A: effect of exposure of the pancreatic acinar cell line PAC 266-6 to proinflammatory cytokines on thiamin uptake. PAC 266-6 were treated with proinflammatory cytokines [hyper IL-6 (100 ng/mL), free IL-6 (100 ng/mL), TNF-α (20 ng/mL), and IL-1β (10 ng/mL)] for 24 h, and then carrier-mediated thiamin uptake was examined. B–E: effect of hyper IL-6 treatment on level of expression of thiamin transporter-1 and -2 (THTR-1 and -2). PAC 266-6 cells were treated with hyper IL-6 and levels of expression of THTR-1 and -2 proteins (B and C, i and ii) and mRNAs (D and E) were examined. Insets: representative Western image for THTR-1 and THTR-2 proteins. Data are means ± SE of 3–4 independent experiments. **P < 0.01; *P < 0.05; NS, not significant.