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. 2020 Nov 4;320(1):G108–G116. doi: 10.1152/ajpgi.00361.2020

Table 1.

Sequence of the primers for amplifying coding regions of thiamin transporters by real-time PCR

Gene Name Forward and Reverse Primers (5′–3′)
h-THTR-1
 Forward GCCAGACCGTCTCCTTGTA
 Reverse TAGAGAGGGCCCACCACAC
h-THTR-2
 Forward TTCCTGGATTTACCCCACTG
 Reverse GTATGTCCAAACGGGGAAGA
h-β-actin
 Forward CATCCTGCGTCTGGACCT
 Reverse TAATGTCACGCACGATTTCC
m-THTR-1
 Forward GTTCCTCACGCCCTACCTTC
 Reverse GCATGAACCACGTCACAATC
m-THTR-2
 Forward TCATGCAAACAGCTGAGTTCT
 Reverse CTCCGACAGTAGCTGCTCA
m-β-Actin
 Forward ATCCTCTTCCTCCCTGGA
 Reverse TTCATGGATGCCACAGGA

THTR-1 and -2, thiamin transporter-1 and -2; h, human; m, mouse.