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. 2021 Apr 27;12:663022. doi: 10.3389/fendo.2021.663022

Figure 3.

Figure 3

Adrenergic innervation of the endocrine pancreas (photomicrographs are from experiments conducted by Dr. Joana Almaca, unpublished data). (A) Mouse pancreatic section immunostained for the sympathetic marker tyrosine hydroxylase (TH, green), the cholinergic marker vesicular acetylcholine transporter (vAChT, red), and DAPI (blue); scale bar 50 µm. (B-B’) Mouse pancreatic section immunostained for TH (green), endothelial marker CD31 (red), and DAPI (blue); scale bars 20 µm (B) and 10 µm (B’). (C) Mouse pancreatic section immunostained for TH (green), glucagon (red), and DAPI (blue); scale bar 10 µm. (D) Mouse pancreatic section immunostained for TH (green), vAChT (red), and DAPI (blue); scale bar 10 µm. TH-positive fibers can be seen along the perivascular space of a large blood vessel (B) and intra-islet capillaries (B’). TH-positive varicosities contact endothelial vascular cells (B’), endocrine α-cells (arrows in C), and neurons within a neuroinsular complex (D). Asterisks denote an alpha cell (in C) and a neuron (in D).