Figure 7.
Potential model for recording Ca2+ neuronal activity in response to islet stimulation in vitro. (A) Schematic representation of the proposed experimental setup, where islets are placed upstream of neurons in a perfusion chamber or a microfluidics device. Pharmacological or optogenetic stimulation of islets will induce release of substances that have a potential to act on downstream neurons. (B) Representative photomicrograph of islets placed in close proximity to the nodose ganglion (NG) in an imaging chamber.