Table 2.

Neuronal markers, antibodies, mouse transgenic lines, and promoters for neuronal labeling.

Innervation type	Marker	Antibodies validated in the pancreas	Promoters/Transgenic lines
Cholinergic (primary parasympathetic, primary sympathetic, intrinsic pancreatic)	ACh		N/A
	vAChT	139103, Synaptic Systems	SLC18A3-cre (PMID: 14502577)
	ChAT		ChAT-eGFP (Jax 007902) ChAT-Cre (Jax 006410)
Adrenergic (secondary sympathetic)	TH	AB152, Millipore	TH-Cre (Jax 008601)
Peptidergic-sensory (vagal afferents, spinal afferents, intrinsic sensory)	SP	MAB356, Millipore	Tac1-Cre (Jax 021877)
	CGRP	BML-CA1134, Enzo	CALCA-Cre/eGFP (Jax 033168)
	TRPV1	Alomone labs ACC-030	TRPV1-Cre (Jax 017769)
	5HT3R		5HT3R-GFP (PMID 19095802) 5HT3R-Flpo (Jax 030755)
	Pirt		Pirt-GCaMP3 (JHU C13628) Pirt-Cre (JHU C13783)
Serotonergic (enteric)	5HT		N/A
Placodal-derived (vagal afferent and efferent)	Phox2b		Phox2b-Cre (Jax 016223)
Neural Crest-derived	Wnt1		Wnt1-Cre (Jax 022137)
Pan-neuronal markers	b3Tub	MRB-435P, Covance	TUBB3-eGFP (MGI:4847518)
	Pgp9.5	#233003, Synaptic Systems	UCHL1-eGFP (Jax 022476)
	Thy1		Thy1-Cre (Jax 006143)
	Snap25		Snap25-GCaMP6 (Jax 025111)
	Synaptophysin (Syp)		Syp promoter RC::FPSit (Jax 030206) Ai34D (Jax 012670)
	Synapsin (Syn)	Cell Signaling Technology 5297 #106011C5, Synaptic Systems	Syn1-Cre (Jax 003966) hSyn - most common AAV promoter on AddGene
	Acetylated tubulin	T6793, Sigma	TUBA1A promoter
	NeuN		RBFOX3 promoter
	S100	ab52642, Abcam	S100b-Cre (Jax 014160)
	NF200	Sigma-Aldrich N4142
	cFos	#226003, Synaptic Systems	TRAP2 (Jax 030323)