Table 2.
Neuronal markers, antibodies, mouse transgenic lines, and promoters for neuronal labeling.
| Innervation type | Marker | Antibodies validated in the pancreas | Promoters/Transgenic lines |
|---|---|---|---|
| Cholinergic (primary parasympathetic, primary sympathetic, intrinsic pancreatic) | ACh | N/A | |
| vAChT | 139103, Synaptic Systems | SLC18A3-cre (PMID: 14502577) | |
| ChAT | ChAT-eGFP (Jax 007902) ChAT-Cre (Jax 006410) |
||
| Adrenergic (secondary sympathetic) | TH | AB152, Millipore | TH-Cre (Jax 008601) |
| Peptidergic-sensory (vagal afferents, spinal afferents, intrinsic sensory) | SP | MAB356, Millipore | Tac1-Cre (Jax 021877) |
| CGRP | BML-CA1134, Enzo | CALCA-Cre/eGFP (Jax 033168) | |
| TRPV1 | Alomone labs ACC-030 | TRPV1-Cre (Jax 017769) | |
| 5HT3R | 5HT3R-GFP (PMID 19095802) 5HT3R-Flpo (Jax 030755) |
||
| Pirt | Pirt-GCaMP3 (JHU C13628) Pirt-Cre (JHU C13783) |
||
| Serotonergic (enteric) | 5HT | N/A | |
| Placodal-derived (vagal afferent and efferent) | Phox2b | Phox2b-Cre (Jax 016223) | |
| Neural Crest-derived | Wnt1 | Wnt1-Cre (Jax 022137) | |
| Pan-neuronal markers | b3Tub | MRB-435P, Covance | TUBB3-eGFP (MGI:4847518) |
| Pgp9.5 | #233003, Synaptic Systems | UCHL1-eGFP (Jax 022476) | |
| Thy1 | Thy1-Cre (Jax 006143) | ||
| Snap25 | Snap25-GCaMP6 (Jax 025111) | ||
| Synaptophysin (Syp) | Syp promoter RC::FPSit (Jax 030206) Ai34D (Jax 012670) |
||
| Synapsin (Syn) | Cell Signaling Technology 5297 #106011C5, Synaptic Systems |
Syn1-Cre (Jax 003966) hSyn - most common AAV promoter on AddGene |
|
| Acetylated tubulin | T6793, Sigma | TUBA1A promoter | |
| NeuN | RBFOX3 promoter | ||
| S100 | ab52642, Abcam | S100b-Cre (Jax 014160) | |
| NF200 | Sigma-Aldrich N4142 | ||
| cFos | #226003, Synaptic Systems | TRAP2 (Jax 030323) |