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. Author manuscript; available in PMC: 2022 May 28.
Published in final edited form as: Cancer Lett. 2021 Feb 25;506:142–151. doi: 10.1016/j.canlet.2021.01.020

Fig. 2. Confined-migrating cells exhibit more radiation resistance than the 2D cultured cells.

Fig. 2.

A. G55 cells were seeded and allowed to migrate in 5 μm width microchannel (top row of 6-well plate) and to grow in 2D (bottom row of 6-well plate); then the plate was irradiated in various doses with a PDMS cover on the top of the 2D wells to control for the potential effect of PDMS on viability. B. Quantitative comparison of cell viability between 2D and confined-migrating cells at different doses of radiation; average ± Std. *p<0.05. N>20 images from at least 3 wells for migrating cells per group; N=6 for the 2D cultured cells per group. C. Representative live/dead images of confined-migrating G55 cells (migrating through 5 × 5 μm microchannels) treated with 0 Gy and 10 Gy radiation. Images were taken 48 hour post-treatment. (Red arrows indicate lined confined-migrating cells inside microchannels; Green arrows indicate 2D cultured cells.)