A-C. Comparison of selected proteins (ABCG2, ALDH and CD44) expression in G55 cells between 2D culture (2D), confined-migrating (M), and 2 days after reseeding confined-migrating cells on the 2D culture (D2). D-F. Comparison expressions of selected proteins (CD133, ABCG2 and ALDH1) in MDA-MB-231 cells between 2D culture (2D), confined-migrating (M), and 2, 3, 4, and 8 days after reseeding confined-migrating cells (D2, D3, D4, and D8). Representative blot images of each marker are shown below their respective graphs. All results were normalized to the total proteins. Average + Std. *p<0.05. All experiments were reproduced.