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. 2021 Apr 24;21(4):foab027. doi: 10.1093/femsyr/foab027

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© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — A) Expression level of Ppgk1- gfp-Tcyc1 integrated into the new sites for cells after cultivation for ca. 60 generations. Expression from EasyClone site XI-2 is shown for comparisson. B) Growth profiles of strains with integrated gfp cassette, compared with non-transformed strain ST7574 as reference (Ref). C) Maximum specific growth rates (µ_max, h⁻¹) for the growth profiles of strains in panel B. Statistical analysis was performed for µ_max values of each strain and reference by using Student's t test (one-tailed, two-sample unequal variances).