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. 2021 Jun;21(3):294–304. doi: 10.17305/bjbms.2020.4701

FIGURE 2.

FIGURE 2

Effects of ANKRD22 knockdown on the growth and proliferation of breast cancer cell lines. (A) ANKRD22 expression levels in four different breast cancer cell lines. (B) ANKRD22 mRNA expression levels in breast cancer cell line MDA-MB-415 after transfection of ANKRD22 shRNA. (C) ANKRD22 protein expression levels in breast cancer cell line MDA-MB-415 after transfection with ANKRD22 shRNA. (D) The effects of ANKRD22 knockdown on MDA-MB-415 cell proliferation by BrdU assay. (E) Colony formation assay was performed to detect the effect of ANKRD22 knockdown on MDA-MB-415 cell colony formation. ANKRD22: Ankyrin repeat domain 22; shRNA: Short hairpin RNA. Results are presented as mean ± SEM, **p < 0.01.