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. 2021 Jun;21(3):294–304. doi: 10.17305/bjbms.2020.4701

FIGURE 4.

FIGURE 4

ANKRD22 knockdown inhibits NuSAP1 expression and regulates Wnt/β-catenin pathway activation. (A) NuSAP1 protein expression levels in MDA-MB-415 cells with ANKRD22 knockdown. (B) β-catenin protein expression level in the cytoplasm and nucleus of MDA-MB-415 cells after ANKRD22 was knocked down. GAPDH and P84 were used as reference proteins in the cytoplasm and nucleus, respectively. ANKRD22: Ankyrin repeat domain 22; NuSAP1: Nucleolar and spindle-associated protein 1; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase. Results are presented as mean ± SEM, **p < 0.01.