Figure 5.

TGN-localized clathrin adaptors are important for delivery of Mup1 and Tat2 to the vacuole after glucose starvation. A) Indicated cells expressing Mup1-GFP from a plasmid were imaged in synthetic complete media and 1 hr after continued glucose withdrawal. B) Indicated cells expressing Tat2-GFP from a plasmid were imaged in synthetic complete media and 1 hr after continued glucose withdrawal. Bars are 5 μm. Chart shows quantification of cell classes, p=values were calculated using a two-tailed unpaired t-test for the null hypothesis that the percentage of cells with vacuolar localization was equal for mutant and wild-type cells, means with standard deviation are displayed, n=3.