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. 2021 May 11;16(5):e0251411. doi: 10.1371/journal.pone.0251411

Table 1. Composition of acrylamide/bis-acrylamide of fabricated polyacrylamide substrates.

PAA hydrogel Acrylamide (%) Bis-Acrylamide (%) Nexp E (kPa)
A 7.5 0.05 - 3.4 *
B 7.5 0.3 6 13 ± 1
C 12 0.14 7 18 ± 1
D 12 0.28 4 37 ± 7
E 12 0.6 6 46 ± 4

Young’s modulus, E, measured averaging the results of force-distance curves obtained in Nexp experiments. More than 1000 curves were analyzed for each PAA composition. Errors represent the standard error (SE).

* estimated by relative concentrations, using as a reference values obtained in Aratyn-Schaus et al., 2010 [10] and the measured E of hydrogel B.