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. Author manuscript; available in PMC: 2021 May 11.
Published in final edited form as: Methods Mol Biol. 2016;1428:127–137. doi: 10.1007/978-1-4939-3625-0_8

Figure 1: Diagram of the alphavirus RNA genome and translation reporter and the host-mimic.

Figure 1:

(A) The construction of the translation reporter from the alphavirus genome. The alphavirus RNA translation reporters has the m7G capped alphavirus 5’NTR, nsP1 CSE with an in-frame fusion to firefly luciferase, alphavirus 3’NTR and polyA tail. (B) The host mimic as an m7G capped 5’NTR (random sequence), Renilla luciferase, 3’NTR (random sequence) and polyA tail.