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Electroporation Conditions for Various Cell Types
| Electroporator | Cell Type | Voltage1 (V) | Capacitance (uF) | Pulse Width (ms) | Pulses | Alphavirus Translation Reporter Concentration (μg) | Host Translation Reporter Concentration (μg) |
|---|---|---|---|---|---|---|---|
| BioRad Electroportor | BHK | 220 | 1000 | N/A2 | 2 | 7.5 | 0.75 |
| MEF | 220 | 1000 | N/A | 2 | 7.5 | 7.5 | |
| C7/10 | 360 | 1000 | N/A | 1 | ND3 | ND | |
| Neon® Electroporator | BHK | 1200 | N/A | 30 | 1 | ND | ND |
| RAW264.7 | 1750 | N/A | 25 | 1 | 7.5 | 0.75 | |
| RAJI | 1375 | N/A | 30 | 1 | ND | ND |
