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. Author manuscript; available in PMC: 2021 May 11.
Published in final edited form as: Cell Rep. 2021 Apr 13;35(2):108979. doi: 10.1016/j.celrep.2021.108979

Figure 5. Prolonged exposure to unpredictable stressors impairs cytokine production in response to αGC.

Figure 5.

(A) B6 mice were subjected to 21 days of chronic variable stress (CVS) involving 3 weekly cycles of exposure to the indicated heterotypic stressors. Control mice remained undisturbed in home cages with food and water supplied ad libitum.

(B and C) At the conclusion of the third cycle, serum CS was measured by ELISA (B), and hepatic and splenic iNKT cells were enumerated (C).

(D) In additional experiments, parallel cohorts of mice (n = 4 per group) were subjected to CVS, or to repeated restraint stress (RRS) involving 1 h of daily physical confinement for 21 consecutive days. Control mice were left undisturbed. At the conclusion of the 21-day period, animals were injected with αGC and assessed for their serum IL-4, IFN-γ, and IL-2 levels at the indicated time points.

Each symbol in (B) and (C) represents a mouse. Error bars denote SEM. Statistical comparisons were conducted using unpaired Student’s t tests (B and C) or two-way ANOVA with Tukey’s correction (D). *p < 0.05, **p < 0.01; NS, not significant.