Fig. 7.

Autophagy reduces the fraction of depolarized mitochondria caused by UVB damage. (A) Confocal images of mesophyll cells expressing MT-GFP and stained with TMRE from WT, atg5 and atg7 untreated control plants or plants 1 d after a 1-h UVB (1.5 W m⁻²) exposure. Green, mitochondrial GFP; magenta, TMRE. Orthogonal projections created from z-stack images are shown. Scale bars = 10 μm. The area indicated by a dashed blue box is expanded to the right of each panel. (B) Number of MT-GFP-labeled or TMRE-labeled mitochondria obtained from the observation described in (A) (� SE, n = 4). (C) Proportion of the number of TMRE particles in MT-GFP particles in (B). Different letters in each graph denote significant differences based on Tukey’s test (P < 0.05).