Fig. 3.

ERK1 and TIC mutants display notable ionomic changes and sensitivity to excess iron. (A) Heat-map showing differences in ion accumulation in shoots from wild-type and CS mutants (n = 15). (B) Induction of ERK1-GFP expression in root endodermis by elevated iron-EDTA. (C) Quantification of ERK1-GFP expression in endodermis by iron-EDTA at different pH (n = 10). Differences between groups were determined by paired t-test, ***P < 0.001. (D) Sensitivity of plants grown in vitro with different iron concentrations. (E) Relative mean fresh weight of plants grown in media with elevated iron-EDTA. (n = 6). Differences between groups were determined by paired t-test, ***P < 0.001.