Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293 FlpIn T-REx HASt-DIM2 | (Wyler et al., 2011) DOI: 10.1261/rna.2325911 | ||
| Cell line (Homo sapiens) | HEK293 FlpIn T-REx HASt-LTV1 | (Wyler et al., 2011) DOI: 10.1261/rna.2325911 | ||
| Cell line (Homo sapiens) | HEK293 FlpIn T-REx RIOK1-StHA | (Widmann et al., 2012) DOI: 10.1091/mbc.E11-07-0639 | ||
| Cell line (Homo sapiens) | HEK293 FlpIn T-REx RIOK1(D324A)-StHA | (Widmann et al., 2012) DOI: 10.1091/mbc.E11-07-0639 | ||
| Transfected construct (human) | si-RPS26 | Eurogentec | 5′-GGACAAGGCCAUUAAGAAA dTdT-3′ | |
| Transfected construct (human) | si-EIF1AD | Eurogentec | 5′-ACCGCAGACAGUAUCAUGAGA-3′ | |
| Transfected construct (human) | si-control | Eurogentec | SR-CL000-005 | |
| Antibody | Anti-DIM2 (rabbit polyclonal) | (Zemp et al., 2009) DOI: 10.1083/jcb.200904048 | WB(1:2000) | |
| Antibody | Anti-ENP1 (rabbit polyclonal) | (Zemp et al., 2009) DOI: 10.1083/jcb.200904048 | WB(1:5000) | |
| Antibody | Anti-HA-HRP (rabbit polyclonal) | Sigma-Roche Applied Science | Cat# MMS-101P RRID:AB_2314672 |
WB(1:1000) |
| Antibody | Anti-NOB1 (rabbit polyclonal) | (Zemp et al., 2009) DOI: 10.1083/jcb.200904048 | WB(1:5000) | |
| Antibody | Anti-RIOK2 (rabbit polyclonal) | (Zemp et al., 2009) DOI: 10.1083/jcb.200904048 | WB(1:5000) | |
| Antibody | Anti-RPS26 (rabbit polyclonal) | Genetex | GTX131193-S | WB(1:1000) |
| Antibody | Anti-RPS19 (rabbit polyclonal) | (This article) | WB(1:5000) | |
| Antibody | Anti-EIF1AD (rabbit polyclonal) | Proteintech | 20528–1-AP | WB(1:2000) |
| Sequence-based reagent | 3′18S probe | (Larburu et al., 2016) DOI: 10.1093/nar/gkw714 | Northern blot probe, 5′-GATCCTTCCGCAGGTTCACCTACG-3′ |
|
| Sequence-based reagent | RNase H_3_Hyb1 (RNA/DNA/RNA) | Eurogentec | 5′-UGUUACGACUUUUACTTCCUCUAGAUAGUCAAGUUC-3′ | |
| Sequence-based reagent | Lad_S oligo | Eurogentec | 5′-TAATACGACTCACTATAGGCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGGGATGAAGATGATGAGCTCGGCAGGTCCTGAGGAGTGATGA-3′ | |
| Sequence-based reagent | Lad_AS oligo | Eurogentec | 5′- AAGGTGAATCAGCACTCAAGATCCTCATCACTCCTCAGGACC-3′ | |
| Sequence-based reagent | Ladder sequence | This article | 5′-UAAUACGACUCACUAUAGGCGUAGGUGAACCUGCGGAAGGAUCAUUAACGGAGCCCGGAGGGCGAGGGAUGAAGAUGAUGAGCUCGGCAGGUCCUGAGGAGUGAUGAGGAUCUUGAGUGCUGAUUCACCUU-3′ |