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. Author manuscript; available in PMC: 2021 May 11.
Published in final edited form as: Trends Biochem Sci. 2020 Nov 30;46(3):225–238. doi: 10.1016/j.tibs.2020.10.006

Figure 4.

Figure 4.

Prp16 in the Spliceosome C Complex.

(A) Overall structure of the yeast C complex (PDB ID 5LJ5) showing the hypothetical path of the 3′ end of the pre-mRNA that would allow for remodeling by Prp16. (B) C complex rotated by 45° with respect to panel A. The U6 small nuclear RNA (snRNA; orange) does not come close to Prp16 and thus can be ruled out as a target of Prp16. (C) A mechanistic model shows that Prp16 pulls the pre-mRNA, dislodging Cwc25 and Yju2 and leading to destabilization of the U2–BP interaction. Abbreviation: BP, branch point.