Table 1.

Mouse anti-human antibodies with conjugated fluorochromes were used in the experiment. Phenotyping was performed in 4 color schemes in three tubes. Each tube included CD56 and CD16 to gate the population of interest: in tube 1, the induction of NK cells to idNK cells and killer immunoglobulin-like receptors were assessed, and in tube 2, ILT expression was checked.

Marker	Fluorochrome	Clone	Isotype control	Manufacturer	The volume of Abs per tube	Tubes
CD56	PE-Cy7	B159	—	Becton Dickinson	5 μl	1, 2
CD16	FITC	3G8	—	Becton Dickinson	20 μl	1, 2
CD158a/KIR2DL	PE	HP-3E4	Mouse BALB/c IgM	Becton Dickinson	20 μl	1
CD159a/NKG2A	APC	Z199	Mouse IgG2b	Beckman Coulter	7 μl	1
CD85j/ILT-2	PE	GHI/75	Mouse IgG2b	Becton Dickinson	20 μl	2
CD85d/ILT-4	Alexa647	287219	Mouse IgG2a, κ	Becton Dickinson	5 μl	2