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. 2021 May 4;2021:6688629. doi: 10.1155/2021/6688629

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Copyright © 2021 Meng Wang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Anticancer effects of berberine (BBR) in gastric cancer cells. (a) AGS and HGC27 cell viability following berberine treatment for 24, 48, and 72 h determined using CCK-8 assay. (b) Colony formation assay showed the clone number in AGS and HGC27 cells treated with 50 μM BBR or control. (c) AGS and HGC27 cell apoptosis after treatment with BBR for 48 h determined using flow cytometry. (d) Mitochondrial membrane potential of AGS cells following treatment with BBR for 48 h and staining with JC-1 probe. Data are presented as the means ± SEM; P value significance: 0 ≤ ^∗∗∗∗ ＜ 0.0001 ≤ ^∗∗∗ ＜ 0.001 ≤ ^∗∗ ＜ 0.01 ≤ ^∗ ＜ 0.05 ≤ ns; ns: not significant.