Table 9.
Methods to verify potential Prdx interactors
| Methods | Prdx1 Interactor |
Prdx2 Interactor |
Prdx3 Interactor | Prdx4 Interactor | Prdx5 Interactors | Prdx6 Interactor |
|---|---|---|---|---|---|---|
| Chromatography | Stomatin [191] | |||||
| Proximity-based immunofluorescence (PLA, etc.) | ANXA2 [31] | πGST [192] | ||||
| Yeast two-hybrid | PDGF-R [193] | |||||
| Co-IP/pulldown | AR [194], TLR4 [195], ASK1 [33], GSTpi-JNK complex [196], PTEN [197], FOXO3 [198], PPP3CA [199], TRAF6 [200], NF-κB [201] | STAT3 [21], tankyrase [202] | GDE2 [162] | Nrf2 [203] | Sumo1 [204], NPM [173] | |
| Co-localization | GDE2 [162] | caspase‐1 [205], GDE2 [162] | Nrf2 [203] | NPM [173] | ||
| X-ray crystallography | Srx [72] | |||||
| Co-incubation SDS-PAGE - MS | caspase‐1 [205], PDI [206], PDIA6 [206], TXNDC5 [206] |