Table 1.
Overview and basic characteristics of 8-oxodG ELISA kits currently available on the market. Information obtained from webpages of individual manufacturers. N.B., We have largely retained the nomenclature used by the manufacturer to describe the kit, which may not be accurate, or indeed correct. Furthermore, the same kit may be distributed by multiple companies, but it is often impossible to establish whether this is the case, if the kit has been re-branded. Where possible, we have aimed to identify this.
| Kit name | Sample type | Sensitivity | Specificity/cross-reactivity/other note | Manufacturer | Kit price | Comment |
|---|---|---|---|---|---|---|
| 8-oxo-dG ELISA Kit | DNA, plasma, urine, saliva samples | 0.57 ng/mL | Cross-reactivity: 8-hydroxydeoxyguanosine 100%, 8-hydroxyguanosine ~30%, 8-hydroxyguanine ~20%, 8-mercatoguanosine ~4%, 8-bromoguanosine CMP, guanosine (Guo), guanine (Gua), 2′-deoxyinosine, N2-methylguanosine | Creative Diagnostics, New York, NY, USA | Upon inquiry | No information on experimental protocol was available at the manufacturer's website |
| New 8-OHdG Check ELISA | Urine from human and animals | 0.5 ng/mL | Antibody N45.1. Specific for 8-OHdG, tested against 8-OHdG analogues: Guo, 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, Gua, O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G. | Japan Institute for the Control of Aging (JaICA), Fukuroi, Shizuoka, Japan | US$ 450 | A commonly used kit that includes the well characterized antibody N45.1. Manufacturer recommends for analysis of urine samples, and was used with our improved methodology for urine. |
| Highly sensitive 8-OHdG Check ELISA | Urine, serum, tissue and cultured cells | 0.125 ng/mL | Antibody N45.1. Specific for 8-OHdG, tested against 8-OHdG analogues: Guo, 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, Gua, O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G. | Japan Institute for the Control of Aging (JaICA), Fukuroi, Shizuoka, Japan | US$ 450 | A kit similar to the “New 8-OHdG Check ELISA”, but with higher sensitivity, manufacturer specifically recommends for analysis of serum, tissue and cultured cells. |
| High Sensitivity 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Assay Kit | Plasma and DNA extracts from cells or tissue | 0.125 ng/mL | Antibody N45.1 (see above). | Northwest Life Science Specialities, Vancouver, WA, USA | US$ 945 | This kit appears to be similar/identical to “Highly sensitive 8-OHdG Check ELISA” by JaICA |
| DNA Damage (8-OHdG) ELISA kit | Cell lysates, plasma, urine | 0.59 ng/mL | Cross-reactivity: 8-hydroxy-2-deoxyguanosine (8-OHdG): 100%. 8-hydroxyguanosine (8-OHG): 23%. 8-hydroxyguanine (8-oxoG): 23%. guanosine: <0.01%. | StressMarq Biosciences Inc., Victoria, BC, Canada |
US$ 399 | No information on the primary antibody. No detailed protocol available. Incubation time of the samples 1 h, but temperature is not specified (probably room temperature). |
| DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit | Urine, plasma, serum, culture media, cell lysates, tissue samples, saliva | 0.30 ng/mL | Cross Reactivity: 8-hydroxy-2′-deoxyguanosine: 100%, 8-hydroxyguanosine: 38%, 8-hydroxyguanine: 23%, guanosine: <0.01% | Cayman Chemical Ann Arbor, MI, USA |
Upon inquiry | This kit is also recommended, by the manufacturer, for detection of oxidatively damaged RNA. A competitive ELISA that uses 8-OH-dG-acetylcholinesterase conjugate. No information on primary antibody provided. |
| 8-hydroxy 2 deoxyguanosine ELISA Kit | Cell culture supernatant, cell lysate, plasma, saliva, serum, tissue extracts, urine | 0.59 ng/mL | 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS | Abcam, Cambridge, UK | € 795 | No information on primary antibody. The manufacturer explicitly mentions discrepancy between ELISA and chromatography. Incubation of the samples with primary antibody 1 h at room temperature. (N.B. Evidence suggests that this will decrease the kit's potential specificity, as incubation at 4 °C overnight improves Ab binding specificity.) |
| DNA Damage (8-OHdG) ELISA kit | Urine, cell culture, plasma and other sample matrices. | 0.59 ng/mL | 8-OHdG antibody used in this assay recognizes both free 8-OHdG and 8-OHdG incorporated into DNA. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results. | Agrisera, Vännäs, Sweden | € 669 | The kit's description suggests similarity with “8-hydroxy 2 deoxyguanosine ELISA Kit” by Abcam. Incubation of the samples with primary antibody 1 h at room temperature (see above comment). |
| 8 Hydroxyguanosine (8-OHdG) ELISA Kit | Urine, cell culture supernatant, serum, plasma, saliva and cell/tissue lysate | 0.59 ng/mL | 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results. | Arigobio, Hsinchu City, ROC, Taiwan | Upon inquiry | The kit description suggests similarity with “8-hydroxy 2 deoxyguanosine ELISA Kit” by Abcam. Incubation of the samples with primary antibody 1 h at room temperature (see above comment). |
| DNA damage ELISA kit | Plasma, seminal fluids, cell lysates, culture supernatants and DNA extracts | 0.59 ng/mL | The DNA Damage ELISA also detects 8-hydroxyguanosine (product of oxidative RNA damage) and 8-hydroxyguanine (product of oxidative DNA damage by hydroxyl radicals). | Enzo Life Sciences, Inc., New York, NY, USA | Upon inquiry | No information on primary antibody. Incubation of the samples with primary antibody for 1 h at room temperature (see above comment). |
| ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG) | Serum, plasma and other biological fluids | 0.74 ng/mL | This assay has high sensitivity and excellent specificity for detection of 8-Hydroxydeoxyguanosine (8-OHdG). No significant cross-reactivity or interference between 8-Hydroxydeoxyguanosine (8-OHdG) and analogues was observed. | Cloud-Clone Corp., Katy, TX, USA | US$ 796 | Primary antibody not specified. Requires incubation of the samples with primary antibody 1 h at 37 °C (see above comment). |
| 8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA Kit | Serum, plasma, tissue homogenates, culture supernatants and other biological fluids | 0.469 ng/mL | Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed. | BioTrend, BioVision Inc., Köln, Germany | Upon inquiry | No information on primary antibody. The samples are incubated with the primary antibody for 45 min at 37 °C (see above comment). |
| ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG) | Serum, plasma and other biological fluids | 0.74 ng/mL | This assay has high sensitivity and excellent specificity for detection of 8-Hydroxydeoxyguanosine (8-OHdG). No significant cross-reactivity or interference between 8-Hydroxydeoxyguanosine (8-OHdG) and analogues was observed. | USCN, Wuhan USCN Business Co., Ltd., Hubei, China | US$ 796 | The kit appears to be identical to “ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG)” by Cloud-Clone Corp. |
| HT 8-oxo-dG ELISA Kit II | Plasma, urine, saliva samples and cultured cells | 0.57 ng/mL | N/A | R&D Systems, Inc., Minneapolis, MN, USA | US$ 962 | Primary antibody not specified. Information on possible interference with “factors” in the samples compromising the assay. Samples incubated with primary antibody 1 h at room temperature (see above comment). |
| The HT 8-oxo-dG ELISA Kit II quantifies 8-hydroxy-2′-deoxyguanosine (8-OHdG) | DNA, plasma, urine and saliva samples | 0.57 ng/mL | N/A | Trevigen, Gaithersburg, MD, USA | US$ 962 | The kit is identical to “HT 8-oxo-dG ELISA Kit II” by R&D Systems, Inc. |
| HT 8-oxo-dG ELISA Kit II | DNA, plasma, urine and saliva samples | 0.57 ng/mL | N/A | Bio-Techne, Minneapolis, MN, USA | US$ 962 | The kit is identical to “HT 8-oxo-dG ELISA Kit II” by R&D Systems, Inc. |
| Human 8-Hydroxyguanine (8-oxo-dG) ELISA Kit | Serum, plasma, cell culture supernatants, cell lysates, tissue homogenates | 2.89 ng/mL | N/A | MyBioSource, San Diego, CA, USA | US$ 405 | A non-standard sandwich ELISA. No information on anti-8-oxodG antibodies provided. Samples incubation 1 h at 37 °C (see above comment). |
| 8-OHdG DNA Damage ELISA | Urine, serum, cells or tissues | 0.10 ng/mL | N/A | Cell Biolabs Inc., San Diego, CA, USA | US$ 820 | No information on primary antibody, no specific samples pre-treatment recommended. Samples should be incubated with primary antibody for 1 h at room temperature (see above comment). |
| 8OHDG ELISA Kit | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | 0.27 ng/mL | N/A | CliniSciences, Nanterre, France | Upon inquiry | The kit is manufactured by Aviva Systems Biology – see below for more information. |
| 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit | Serum, plasma and other biological fluids | 0.94 ng/mL | N/A | Elabscience, Houston, TX, USA | US$ 495 | A competitive format that uses biotin-avidin-horseradish peroxidase for detection. Samples incubation: 45 min at 37 °C (see above comment). |
| 8-Hydroxy-2' -Deoxyguanosine (8-OHdG) ELISA Kit | Serum, plasma and other biological fluids. | 0.94 ng/mL | N/A | Abbexa Ltd, Cambridge, UK | € 669 | The kit appears to be identical with “8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit” by Elabscience |
| Human 8-Hydroxydeoxyguanosine (8-OHdG) ELISA kit | Serum, urine | 3.12 ng/mL | N/A | CUSABIO, Houston, TX, USA | Upon inquiry | No information on primary antibody. Np specific instructions for urine samples processing, apart from centrifugation. The samples are incubated with primary antibody for 30 min at 37 °C (see above comment). |
| DNA Damage (8-OHdG) ELISA Kit | Cell lysates, plasma, urine | 0.59 ng/mL | 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results. | BOSTERBIO, Pleasanton, CA, USA | US$ 390 | The kit description suggests similarity with “8-hydroxy 2 deoxyguanosine ELISA Kit” by Abcam. |
| 8OHDG ELISA Kit | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | 0.27 ng/mL | N/A | Aviva Systems Biology, San Diego, CA, USA | US$ 559 | The clone of primary antibody not specified. No specific information on urine samples processing. Kit uses 8-OHdG-biotin complex that should be incubated with samples for 1 h at room temperature (see above comment). |
| 8-OHdG Assay Kit | Tissue, serum, plasma or urine samples | NA | N/A | Oxford Biomedical Research, Oxford, MI, UK | US$ 1130 |
No information on experimental protocol available at the manufacturer's website |
N/A, information not available.