Table 3.
Summary Table
| Condition (FBS % - Rosi μM – Duration) | Number of CEs with no significant difference across all conditions | Number of CEs where given condition is superior to all other conditions | Number of CEs where given condition is equivalent to the superior condition (p > 0.05) | Number of CEs where given condition is inferior to the superior condition (p < 0.05) | Total Number of CEs | Percentage of CEs that responded optimally to each condition |
|---|---|---|---|---|---|---|
| 2-DMSO-2d | 10 | 2 | 14 | 8 | 34 | 76.5% |
| 2-DMSO-5d | 10 | 2 | 11 | 11 | 34 | 67.7% |
| 2-20-2d | 10 | 0 | 16 | 8 | 34 | 76.5% |
| 2-20-5d | 10 | 0 | 14 | 10 | 34 | 70.6% |
| 2-50-2d | 10 | 10 | 8 | 6 | 34 | 82.4% |
| 2-50-5d | 10 | 3 | 14 | 7 | 34 | 79.4% |
| 10-DMSO-2d | 10 | 0 | 9 | 15 | 34 | 55.9% |
| 10-DMSO-5d | 10 | 1 | 8 | 15 | 34 | 55.9% |
| 10-20-2d | 10 | 0 | 11 | 13 | 34 | 61.8% |
| 10-20-5d | 10 | 1 | 8 | 15 | 34 | 55.9% |
| 10-50-2d | 10 | 2 | 14 | 8 | 34 | 76.5% |
| 10-50-5d | 10 | 3 | 11 | 10 | 34 | 70.6% |
Each culture condition was evaluated to determine the number of cholesteryl esters (CEs) that responded optimally. Culture conditions are abbreviated as FBS % – Rosiglitazone (μM) – Duration. CEs are listed with two numbers. The two numbers denote the number of carbons and double bonds in the acyl chains, respectively. n = 4 for each culture condition.
A total of 34 CEs, as shown in Column E, met the criteria for analysis in this study. Column A shows that 10 CEs did not vary across all twelve culture conditions. The definition of a “superior” condition in Column B is dependent on a given CE’s saturation level. For saturated and monounsaturated CEs, a condition was deemed superior if it yielded the highest expression relative to the other conditions. For polyunsaturated CEs, a condition was considered superior if it yielded the lowest expression relative to the other conditions. If a given condition was not the “superior” condition, then one of two other outcomes were possible: it could either have been statistically equivalent to the superior condition (Column C) or statistically inferior to the superior condition (Column D). The final column represents the percentage of CEs that responded optimally to each condition. Culturing with 50 uM rosiglitazone and 2% FBS for 2 days optimized 82.4% of CEs and was considered superior for 10 of 34 (29.4%) of CEs.