TABLE 1.
Antibodies Used in this Study
| Antibody Name | Immunogen | Antibody Info | Dilution |
|---|---|---|---|
| Anti-Vesicular glutamate transporter 2 (VGLUT2) | KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat VGLUT2. | EMD Millipore Corporation; Cat# AB2251; RRID:AB_1587626; Guinea Pig (Polyclonal) | 1:2,500 |
| Anti-Vesicular glutamate transporter 1 (VGLUT1) | Synthetic peptide from rat VGLUT1 protein with no overlap to VGLUT2. | EMD Millipore Corporation; Cat# AB5905; RRID:AB_2301751; Guinea Pig (Polyclonal) | 1:5,000 |
| Anti-Choline Acetyltransferase (ChAT) | A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. | EMD Millipore Corporation; Cat# AB5042; RRID: AB_91650; Rabbit (Polyclonal) | 1:2,000 |
| Anti-Glutamic acid decarboxylase 67 (GAD67) | KLH-conjugated synthetic peptide corresponding to a portion of amino acids 1-100 of human GAD67. | Novus Biologicals; Cat# NB100-56385; RRID: AB_838284; Rabbit (polyclonal) | 1:2,000 |
| Anti-Glutamic acid decarboxylase 65 (GAD65) | KLH-conjugated linear peptide corresponding to human GAD65 | Millipore Cat# AB5082, RRID:AB_2107925; Rabbit (polyclonal) | 1:1,000 |
| Secondary Antibodies | |||
| Biotin anti-Guinea Pig IgG | n/a | Vector; Cat# BA-7000; Goat (polyclonal) | 1:50 |
| Biotin anti-Rabbit IgG | n/a | Vector; Cat# BA-1000; Goat (polyclonal) | 1:50 |
| Alexa Flour 488 anti-Rabbit IgG | n/a | ThermoFisher Scientific; CAT# A32731; Goat (polyclonal) | 1:50 |