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. 2021 May 11;12:2683. doi: 10.1038/s41467-021-22993-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a CG, CHG, and CHH methylation levels over TEs in pericentromeres and chromosome arms in WT and h2a.w-2. TEs were aligned at the 5′ (left dashed line) and 3′ end (right dashed line), and sequences 3 kb upstream or downstream were included, respectively. Average methylation over 100 bp bins is plotted. b CHG and CHH methylation levels over H2A.W peaks in the chromosome arms and pericentromeres. c Locally weighted scatterplot smoothing (LOESS) fit of CHG and CHH methylation levels in WT and h2a.w-2 calculated in 50 bp windows and plotted against TE size. d Kernel density plots of CHH DNA methylation differences between h2a.w-2 and WT at CMT2-dependent and DRM1/2-dependent regions.