Fig. 3. Loss of H2A.W alters heterochromatin organization and accessibility.

a Representative images of DAPI-stained WT (Col-0) and h2a.w-2 leaf interphase nuclei. Two independent experiments were performed with similar results. Scale bar is 5 µm. b Locally weighted scatterplot smoothing (LOESS) fit of ATAC-seq read depth averaged in 1 kb bins across chromosome 3 in WT and h2a.w-2. Average of two replicates shown. The gray rectangle indicates the location of pericentromeric heterochromatin. c Smoothing spline fit (50 degrees of freedom) of WT H2A.W levels (log₂ ChIP-seq H2A.W/H3) and of ATAC-seq read depth (CPM normalized) in WT and h2a.w-2 in 1 kb windows plotted against WT H3K9me2 level. d ATAC-seq read depth over H2A.W peaks in pericentromeric heterochromatin. Average of two replicates shown.