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. 2021 May 11;12:2683. doi: 10.1038/s41467-021-22993-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Locally weighted scatterplot smoothing (LOESS) fit of H1 levels averaged in 1 kb bins across chromosome 3 in WT and h2a.w-2. Average of two replicates shown. b Metaplots of average H1 levels from two replicates over H2A.W peaks in pericentromeres and the chromosome arms (top panel). Plots over randomly shuffled peaks within the chromosome arms and pericentromeres are shown for comparison (bottom panel). c Smoothing spline fits (50 degrees of freedom) of change in H1 levels (h2a.w-2 minus WT; log₂ ChIP/H3) in 1 kb windows plotted against WT H2A.W level. Source data underlying Fig. 5a are provided as a Source Data file.