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. 2021 May 11;12:2683. doi: 10.1038/s41467-021-22993-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative images of DAPI-stained WT (Col-0), h2a.w-2, h1 and h1 h2a.w leaf interphase nuclei (left; scale bar is 5 µm) and of 3-week-old plants of the same genotypes (right; scale bar is 1 cm). Nuclear preparation and analysis were independently repeated three times with similar results. b Quantification of the relative chromocenter fraction in WT (Col-0), h2a.w-2, h1 and h1 h2a.w nuclei. Number of analyzed nuclei are indicated on the top. Whiskers indicate 1.5X IQR. Outliers are represented by circles. Relative chromocenter fraction in h1 and h1h2a.w show statistically significant difference (P = 4.4e-15, unpaired Mann–Whitney test). c ATAC-seq read depth over H2A.W peaks in pericentromeric heterochromatin. Average of two replicates shown. d Heat maps of average ATAC-seq read depth over H2A.W peaks in pericentromeres ranked based on level H2A.W signal in WT. e Locally weighted scatterplot smoothing (LOESS) fit of WT H2A.W levels (log₂ ChIP/H3; top panel) and changes in chromatin accessibility (mutant / WT; ATAC-seq read depth) in 50 bp windows plotted against TE size.