Table 3.
Results of Dunn’s Test assessing the impact of symbiont infection status, aromatic amino acid supplementation, and glyphosate exposure on cuticle traits (thickness and melanization) and symbiont titers in one-week-old O. surinamensis adults.
| Z | r | Lower 95% | Δ median | Upper 95% | p | Adjusted p | |
|---|---|---|---|---|---|---|---|
| Cuticle thickness | |||||||
| A–B | 1.98 | 0.45 | 0.49 | 1.64 | 3.13 | 4.75E−02 | 1.33E−01 |
| A–C | 3.48 | 0.84 | 1.95 | 3.41 | 5.45 | 5.08E−04 | 4.74E−03 |
| B–C | 1.64 | 0.39 | −0.08 | 1.70 | 3.68 | 1.01E−01 | 2.01E−01 |
| A–D | 1.50 | 0.36 | −0.80 | 1.30 | 3.98 | 1.35E−01 | 2.36E−01 |
| B–D | −0.39 | −0.09 | −2.92 | −0.45 | 2.33 | 6.99E−01 | 8.16E−01 |
| C–D | −1.92 | −0.48 | −5.11 | −2.10 | 0.36 | 5.43E−02 | 1.38E−01 |
| A–E | 0.77 | 0.17 | −0.65 | 0.92 | 2.42 | 4.41E−01 | 5.88E−01 |
| B–E | −1.29 | −0.28 | −2.59 | −0.83 | 0.77 | 1.97E−01 | 3.06E−01 |
| C–E | −2.89 | −0.66 | −5.24 | −2.56 | −0.74 | 3.85E−03 | 2.69E−02 |
| D–E | −0.82 | −0.19 | −3.79 | −0.58 | 2.15 | 4.12E−01 | 5.77E−01 |
| A–F | 2.05 | 0.47 | 0.37 | 2.06 | 3.90 | 4.08E−02 | 1.27E−01 |
| B–F | 0.07 | 0.01 | −1.44 | 0.41 | 2.14 | 9.48E−01 | 9.48E−01 |
| C–F | −1.58 | −0.37 | −4.00 | −1.24 | 0.66 | 1.14E−01 | 2.13E−01 |
| D–F | 0.45 | 0.11 | −2.39 | 0.71 | 3.46 | 6.54E−01 | 7.96E−01 |
| E–F | 1.36 | 0.30 | −0.52 | 1.12 | 3.33 | 1.74E−01 | 2.87E−01 |
| A–G | 4.26 | 0.98 | 3.17 | 4.61 | 6.20 | 2.02E−05 | 5.64E−04 |
| B–G | 2.34 | 0.52 | 1.25 | 2.93 | 4.36 | 1.91E−02 | 7.63E−02 |
| C–G | 0.57 | 0.13 | −0.94 | 1.02 | 2.83 | 5.70E−01 | 7.25E−01 |
| D–G | 2.60 | 0.61 | 0.81 | 3.50 | 5.64 | 9.41E−03 | 4.39E−02 |
| E–G | 3.69 | 0.81 | 2.07 | 3.56 | 5.53 | 2.24E−04 | 3.13E−03 |
| F–G | 2.28 | 0.51 | 0.84 | 2.57 | 4.71 | 2.27E−02 | 7.95E−02 |
| A–H | 1.70 | 0.39 | −0.11 | 1.60 | 3.18 | 8.99E−02 | 1.94E−01 |
| B–H | −0.29 | −0.07 | −1.79 | −0.21 | 1.42 | 7.69E−01 | 8.28E−01 |
| C–H | −1.92 | -0.45 | −4.15 | −2.07 | −0.21 | 5.50E−02 | 1.28E−01 |
| D–H | 0.11 | 0.03 | −2.38 | 0.03 | 2.70 | 9.13E-01 | 9.46E−01 |
| E–H | 0.99 | 0.22 | −1.16 | 0.65 | 2.58 | 3.22E-01 | 4.74E−01 |
| F–H | −0.36 | −0.08 | −2.57 | −0.60 | 1.72 | 7.19E−01 | 8.06E−01 |
| G–H | −2.64 | −0.59 | −4.85 | −3.18 | −1.51 | 8.35E−03 | 4.67E−02 |
| Cuticle melanization | |||||||
| A–B | 0.15 | 0.03 | −0.60 | 0.06 | 0.71 | 8.83E−01 | 9.51E−01 |
| A–C | 3.32 | 0.74 | 0.60 | 1.34 | 2.03 | 8.91E−04 | 4.99E−03 |
| B–C | 3.18 | 0.71 | 0.60 | 1.29 | 2.02 | 1.49E−03 | 6.95E−03 |
| A–D | 0.1 | 0.02 | −0.66 | 0.03 | 0.69 | 9.22E-01 | 9.56E-01 |
| B–D | −0.05 | −0.01 | −0.70 | −0.02 | 0.69 | 9.63E−01 | 9.63E−01 |
| C–D | −3.14 | −0.72 | −2.06 | −1.33 | −0.59 | 1.70E−03 | 6.82E−03 |
| A–E | 0.67 | 0.15 | −0.41 | 0.25 | 0.91 | 5.01E−01 | 6.38E−01 |
| B–E | 0.53 | 0.12 | −0.42 | 0.24 | 0.70 | 5.98E−01 | 6.98E−01 |
| C–E | −2.65 | −0.59 | -1.83 | −1.12 | −0.46 | 8.04E−03 | 2.81E−02 |
| D-E | 0.56 | 0.13 | −0.45 | 0.23 | 0.88 | 5.77E−01 | 7.02E−01 |
| A–F | 2.35 | 0.53 | 0.16 | 0.90 | 1.66 | 1.88E−02 | 5.86E−02 |
| B–F | 2.2 | 0.49 | 0.14 | 0.86 | 1.63 | 2.76E−02 | 7.74E−02 |
| C–F | −0.97 | −0.22 | −1.19 | -0.45 | 0.27 | 3.29E−01 | 4.61E−01 |
| D–F | 2.19 | 0.50 | 0.11 | 0.82 | 1.64 | 2.86E−02 | 6.67E−02 |
| E–F | 1.68 | 0.38 | −0.11 | 0.64 | 1.49 | 9.37E−02 | 1.54E−01 |
| A–G | 4.16 | 0.93 | 1.02 | 1.76 | 2.38 | 3.17E−05 | 8.89E−04 |
| B–G | 4.01 | 0.90 | 0.97 | 1.69 | 2.40 | 5.96E−05 | 8.34E−04 |
| C–G | 0.84 | 0.19 | −0.38 | 0.42 | 1.10 | 4.02E−01 | 5.36E−01 |
| D–G | 3.95 | 0.91 | 1.01 | 1.69 | 2.39 | 7.73E−05 | 7.21E−04 |
| E–G | 3.49 | 0.78 | 1.04 | 1.40 | 2.18 | 4.86E−04 | 3.40E−03 |
| F–G | 1.81 | 0.40 | −0.05 | 0.76 | 1.56 | 6.99E−02 | 1.22E−01 |
| A–H | 2.19 | 0.49 | 0.08 | 0.85 | 1.65 | 2.83E−02 | 7.21E−02 |
| B–H | 2.05 | 0.46 | 0.04 | 0.84 | 1.53 | 4.07E−02 | 8.77E−02 |
| C–H | −1.13 | -0.25 | −1.22 | −0.48 | 0.32 | 2.58E−01 | 3.80E−01 |
| D–H | 2.04 | 0.47 | 0.01 | 0.79 | 1.60 | 4.16E−02 | 8.33E−02 |
| E–H | 1.52 | 0.34 | −0.17 | 0.68 | 1.43 | 1.28E−01 | 1.99E−01 |
| F–H | −0.16 | −0.04 | −0.83 | 0.01 | 0.85 | 8.76E−01 | 9.81E−01 |
| G–H | −1.97 | −0.44 | −1.62 | −0.79 | −0.18 | 4.90E−02 | 9.15E−02 |
| Symbiont 16S titer | |||||||
| A–B | 1.01 | 0.23 | 20,211 | 149,112.5 | 286891 | 3.10E−01 | 3.62E-01 |
| A–C | 3.93 | 0.88 | 275,297 | 380,359 | 546,505 | 8.61E−05 | 3.44E−04 |
| B–C | 2.91 | 0.65 | 101162 | 259,656.5 | 360,640 | 3.57E−03 | 9.10E−03 |
| A–D | 1.51 | 0.35 | 93,032 | 218,182.5 | 356,475 | 1.31E−01 | 1.94E−01 |
| B–D | 0.52 | 0.12 | −56,233 | 75,219 | 198341 | 6.02E-01 | 6.49E−01 |
| C–D | −2.32 | -0.53 | −274,985 | -186004.5 | −85,291 | 2.06E-02 | 3.84E−02 |
| A–E | 2.48 | 0.55 | 183,686 | 308,129 | 417,996 | 1.29E−02 | 2.79E−02 |
| B–E | 1.47 | 0.33 | 59,841 | 134,995.5 | 26,9973 | 1.41E−01 | 1.97E−01 |
| C–E | −1.44 | -0.32 | −176,605 | −114,384 | −16,320 | 1.49E−01 | 1.99E−01 |
| D–E | 0.91 | 0.21 | 658 | 70,294.5 | 171,885 | 3.62E−01 | 4.05E−01 |
| A–F | 4.07 | 0.91 | 279,605 | 401,114 | 545,438 | 4.64E−05 | 2.60E−04 |
| B–F | 3.06 | 0.68 | 137,507 | 259,587.5 | 366,228 | 2.21E−03 | 6.20E−03 |
| C–F | 0.15 | 0.03 | −66,054 | −46 | 68,617 | 8.80E−01 | 8.83E−01 |
| D–F | 2.46 | 0.56 | 88,604 | 185,935.5 | 273,826 | 1.39E−02 | 2.79E−02 |
| E–F | 1.59 | 0.36 | 26,819 | 114,969 | 176,630 | 1.12E−01 | 1.84E−01 |
| A–G | 5.36 | 1.20 | 310,395 | 456,309 | 550,371 | 8.14E−08 | 1.14E−06 |
| B–G | 4.35 | 0.97 | 244,565 | 288,187 | 432,302 | 1.35E−05 | 9.50E−05 |
| C–G | 1.44 | 0.32 | −9 | 10,947 | 80,717 | 1.50E−01 | 1.91E−01 |
| D-G | 3.71 | 0.85 | 182,731 | 216,825.5 | 306,114 | 2.04E−04 | 7.14E−04 |
| E–G | 2.88 | 0.64 | 114,693 | 163089.5 | 183,514 | 3.98E−03 | 9.30E−03 |
| F–G | 1.29 | 0.29 | 3 | 11,905 | 89,556 | 1.96E−01 | 2.39E−01 |
| A–H | 5.61 | 1.25 | 318,871 | 45,5935.5 | 557,535 | 2.05E−08 | 5.74E−07 |
| B–H | 4.59 | 1.03 | 253,041 | 294,578 | 432,331 | 4.34E–06 | 4.05E−05 |
| C–H | 1.68 | 0.38 | 17 | 10,980 | 80,746 | 9.28E−02 | 1.62E−01 |
| D–H | 3.95 | 0.91 | 181,983 | 216,855.5 | 306,115 | 7.78E−05 | 3.63E−04 |
| E–H | 3.12 | 0.70 | 115,217 | 162,716 | 189,619 | 1.79E−03 | 5.57E−03 |
| F–H | 1.53 | 0.34 | 5 | 11938 | 89585 | 1.24E−01 | 1.94E−01 |
| G–H | 0.24 | 0.05 | −775 | 18.03559 | 8514 | 8.07E−01 | 8.37E−01 |
Significant results (adjusted p < 0.05) are highlighted in bold. Z-test statistic, effect size r, lower and upper boundary of 95% confidence intervals and difference of medians, unmodified and Benjamini–Hochberg corrected p-values are reported. Significant results (P.adj < 0.05) are highlighted in bold. A: Sym; B: Sym + 0.1% G; C: Sym + 1% G; D: Sym + 1% AA; E: Sym + 1% AA + 0.1% G; F: Sym + 1% AA + 1% G; G: Apo; H: Apo + 1% AA.