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. 2021 May 11;12:2637. doi: 10.1038/s41467-021-22898-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a RTX-321 expression by anti-IL-12, anti-4-1BBL, and anti-β2M staining. b Representative flow plots showing TCR expression of E7-TCR cells compared with untransduced T cells before co-culture. c–h E7-TCR cells (duplicates from three donors) were incubated with RCT-CTRL, RCT-HPV, RCT-4-1BBL, RCT-IL-12, RCT-HPV-4-1BBL, RCT-4-1BBL-IL-12, RTX-321, or RCT-CMV-4-1BBL-IL-12. The Nur77⁺% at 2 h (c), CD69⁺% at 24 h (d), and 4-1BB⁺% on day 5 (e) of E7-TCR cells. f Representative flow plots showing E7-TCR⁺ expression of RCT-CTRL- and RTX-321-treated E7-TCR cells on day 5. Fold expansion of E7-TCR cells and untransduced CD8⁺ T cells over media-treated controls on day 5. g The granzyme B⁺ % of E7-TCR cells on day 1 and IFNγ concentration in the supernatant on day 5. h T_CM and T_EM of E7-TCR cell numbers on day 5. One-way ANOVA compared to RCT-CTRL; Nur77⁺% (c), CD69⁺% (d), and 4-1BB⁺% (e): RCT-HPV, RCT-HPV-4-1BBL, or RTX-321 P < 0.0001; CD8 T-cell expansion (f): RTX-321 E7-TCR P < 0.0001, untransduced P = 0.0062; granzymeB⁺% (g): RTX-321 P = 0.0021; IFNγ secretion (g): RTX-321 P < 0.0001; T_CM number (h): RCT-HPV-4-1BBL P = 0.011, RTX-321 P < 0.0001; T_EM number (h): RTX-321 P < 0.0001. Data are depicted as mean ± s.d and are representative of two independent experiments. Source data are provided as a Source Data file.