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. 2021 May 11;11:10039. doi: 10.1038/s41598-021-89409-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Wolbachia whole-body density increases subsequent to field establishment. Wolbachia whole-body density was molecularly assayed in whole-body extracts from colony (grey) and Jurujuba samples (green), collected over the post-release phase. Density or titration levels (vertical axis) are relative quantification indexes, reflecting the copy number ratio between wMel WD0513 and the endogenous ribosomal maker RPS17. Data were aggregated in 12-weeks pools and represented by violin plots, with medians and quartiles in solid and dashed red lines, respectively. Statistical inferences were performed by the non-parametric Kruskal–Wallis test followed by Dunn’s post-hoc multiple comparisons. Asterisks highlight differences between groups, considering a significance level (α) equal to 0.05, and its numbers reflects probability ranges: *P < 0.05, **P < 0.01, ***P < 0.001. Graph and statistics were made with GraphPad Prism 8 (https://www.graphpad.com).